biosyn immunocyanin products
The active ingredient, immunocyanin, consists of a stable mixture of two types of protein subunits from native Keyhole Limpet Hemocyanin (KLH). Native KLH is isolated from the hemolymph of the marine mollusk Megathura crenulata using biosyn proprietary non-lethal technology.
The native complex molecule has a molecular weight of 8-32 Million Dalton, and consists of at least 20 subunits. There are two immunologically distinct subunit types, which are composed of eight functional units (FU) respectively. Each FU contains a binuclear copper binding site that reversibly binds molecular oxygen. The molecular weight of the subunits is 400 kDa (kilo Dalton) each.
Physicochemical and biochemical properties of the immunocyanin are as follows:
- Soluble in aqueous buffer at pH 8-10
- Reassociation of subunits occurs at pH 7.5 or below in the presence of calcium and magnesium
- Copper to protein ratio is 1.8?2.5 x 10-3
- UV absorption maxima at 279 nm and 347 nm
- Reversible binding of oxygen
- Isoelectric point (pI) ~ pH 5
- Electrophoretic mobility: In native conditions, the two KLH subunits migrate at 360,000 Dalton (KLH2) and 390,000 Dalton (KLH1). Under denaturing conditions, one band is apparent, migrating at approximately 370,000 - 380,000 Dalton
- Chromatographic size characterization results in a single peak at MW 420 kDa (using Superose? 6)
- The protein demonstrates an apparent sedimentation constant of 14S